Journal: STAR Protocols

Article Title: Protocol for nuclear dissociation of the adult C. elegans for single-nucleus RNA sequencing and its application for mapping environmental responses

doi: 10.1016/j.xpro.2023.102756

Figure Lengend Snippet:

Article Snippet: 96 well flat-bottom plates , Genesee Scientific , # 25-104.

Techniques: Recombinant, Protease Inhibitor, Software, Pore Size, Transferring

Journal: medRxiv

Article Title: Bio-Rad and QIAGEN digital PCR platforms provide equivalent quantification for wastewater-based SARS-CoV-2 surveillance

doi: 10.64898/2026.01.20.26344437

Figure Lengend Snippet: Quantitative comparison of SARS-CoV-2 targets between Bio-Rad QX200 ddPCR and QIAGEN QIAcuity dPCR platforms across all concentration ranges. Scatter plots showing hyperwelled (merged triplicate) concentrations for (A) N1 gene target and (B) N2 gene target. Each point represents a single wastewater sample (n = 95) plotted as log 10 copies L -1 on both axes. Samples are color-coded by concentration bin: high (red, 5×10 4 to 5×10 5 copies L -1 , n=31), medium (orange, 5×10 3 to 5×10 4 copies L -1 , n=32), and low (blue, 1×10 3 to 5×10 3 copies L -1 , n=31). Solid black line represents perfect 1:1 agreement; dashed line showed linear regression fit. Linear regression equations, R 2 values, and 95% confidence intervals (gray shading) are displayed for each target. Mean absolute differences between platforms: N1 = 0.10 log copies L -1 , N2 = 0.06 log copies L -1 . All correlations significant at p < 0.001.

Article Snippet: Total nucleic acids were eluted in 100μL of Buffer AE (19077, QIAGEN, Germantown, MD) and transferred into 96-Well ddPCR Plates (12001925, Bio-Rad Laboratories).

Techniques: Comparison, Concentration Assay